Developement of serum-free media in CHO-DG44 cells using a central composite statistical design
نویسندگان
چکیده
منابع مشابه
Developement of serum-free media in CHO-DG44 cells using a central composite statistical design.
A serum free medium was developed for the production of recombinant antibody against Botulinum A (BoNTA) using dihydrofolate reductase deficient Chinese Hamster Ovary Cells (CHO-DG44) in suspension culture. An initial control basal medium was prepared, which was similar in composition to HAM's F12: IMDM (1:1) supplemented with insulin, transeferrin, selenium and a lipid mixture. The vitamin con...
متن کاملLipase-catalyzed synthesis of isoamyl acetate in free-solvent media– optimization using a central composite design
The catalytic action of two non commercial lipases (E.C. 3.1.1.3) in the synthesis of isoamyl acetate in a solvent-free system, were tested using an experimental design in order to better understand the relationship between the reaction variables. Yields ranging from 67 to 77 % molar conversion were achieved. The synthesis of isoamyl acetate catalyzed by the chosen lipase (Rhizopus sp.) was opt...
متن کاملGeneration of a serum free CHO DG44 cell line stably producing a broadly protective anti-influenza virus monoclonal antibody
Because of the broad neutralization and in vivo protection across influenza A and influenza B virus strains, monoclonal antibody CR9114 is widely used in influenza virus research as a positive control in many experiments. To produce amounts sufficient for the demand requires regular transient transfections, resulting in varying yield as well as differing batch to batch quality. Here, we report ...
متن کاملEffects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells a New Insight into Amino Acid Profiles
Background: The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development however, their role on metabolic pathway is not well understood. Methods: In the present study, the effect of different...
متن کاملEXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN BONE MARROW MESENCHYMAL STEM CELLS
Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytotechnology
سال: 2007
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-007-9074-3